Recombinant Human CDK6/CyclinD3 (N-His tag) | Z500076

abm | Recombinant Human CDK6/CyclinD3 (N-His tag) | Z500076

CDK6 is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors . The CDK6 kinase activity is detected in mid-G1 phase of the cell cycle and is responsible for the phosphorylation and regulation of the activity of tumor suppressor protein Rb. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivities in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein .

Functions:

The specific activity of CDK6/CyclinD3 was determined to be 36 nmol/min/mgas per activity assay protocol using protein substrate Rb (773-928).

Organism:

Human

Source:

Insect cells

Alias:

PLSTIRE, MGC59692 /CCND3

Gene Symbol:

CDK6/CyclinD3

Gene ID:

N/A

Accession:

NM_001259, NM_001760

Formulation:

50mM NaPhosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.2mM DTT, 25% glycerol.

Solubility:

N/A

Appearance:

Liquid

Molecular Weight (kDa):

40

Molecular Weight 2 (kDA):

35

Molecular Weight 3 (kDA):

N/A

Purity:

70% - 90%

Concentration:

Shipping Condition :

Dry Ice

Storage Condition:

Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.