Recombinant Human CXCL14 (BRAK) | Z102379

abm | Recombinant Human CXCL14 (BRAK) | Z102379

Chemokine (CXC motif) ligand 14, also known as breast and kidney-expressed chemokine (BRAK), is a CXC chemokine that is a potent chemoattractant for neutrophils but not for T-cells, B-cells, monocytes or granulocytes. Mature CXCL14 has many conserved features of the CXC family and is 30% homologous to the MIP-α and MIP-β sequences, but differs in that it has an additional five amino acid sequence in between the third and fourth cysteines and a short N-terminus. It is usually highly expressed near its fibroblast source and in epidermal fibroblasts and keratinocytes of skin, but is absent from cancer cells.

Functions:

The ED50 is 0.25-1.25 ng/mL as determined by its ability to induce calcium flux in activated monocytes.

Organism:

Human

Source:

E. coli

Alias:

Chemokine BRAK, MIP-2G, Small-inducible cytokine B14

Gene Symbol:

CXCL14

Gene ID:

9547

Accession:

O95715

Formulation:

Lyophilized from a 0.2µm filtered solution containing 20mM Tris pH 8.5 and 1M NaCl

Solubility:

A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Appearance:

Lyophilized Powder

Molecular Weight (kDa):

N/A

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

>95% as determined by SDS-PAGE

Concentration:

<1.0 EU/μg of recombinant protein as determined by the LAL method

Shipping Condition :

Ambient Temperature

Storage Condition:

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.