Recombinant Human IGF1 | Z100385

abm | Recombinant Human IGF1 | Z100385

The Insulin Growth Factors belong to the Insulin gene family, the prototypic members of which include insulin and relaxin. Also known as somatomedin C, IGF1 is known to stimulate the proliferation of a wide range of cells including muscle, bone, and those of the cartilage tissue. A dominant effector of the growth hormone, mature IGF1 is generated by proteolytic removal of the N- and C- terminal pro-peptide regions. Mature human IGF1 shares 94% and 96% amino acid sequence identity with mouse and rat IGF1, respectively, while exhibiting cross-species activity. Circulating IGF1 is produced by hepatocytes while local IGF1 is produced by various other tissues in which it exerts paracrine effects. Recombinant Human IGF1 is a globular, 7.7 kDa protein that contains 3 native intra-molecular disulfide bonds.

Functions:

The ED50 as determined by a cell proliferation assay using MCF-7 cells was found to be ≤20 ng/ml

Organism:

Human

Source:

E. coli

Alias:

IGF-I, Mechano growth factor, MGF, Somatomedin-C, IBP1
Recombinant Human Insulin Like Growth Factor-I (IGF1)

Gene Symbol:

IGF1

Gene ID:

3479

Accession:

P01343

Formulation:

Lyophilized from a 0.2 μm filtered solution in sodium phosphate (pH 6.5) and 150mM NaCl

Solubility:

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Appearance:

Lyophilized Powder

Molecular Weight (kDa):

7,7

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

>95% as determined by SDS-PAGE

Concentration:

<1.0 EU/μg of recombinant protein as determined by the LAL method

Shipping Condition :

Ambient Temperature

Storage Condition:

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.