Recombinant Human IKK beta (N-GST tag) | Z500177

abm | Recombinant Human IKK beta (N-GST tag) | Z500177

IKKß is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex. The activity of IKKß is stimulated by TNF and IL1 and IKKß forms a heterodimer that interacts with NIK . Overexpression of catalytically inactive IKKß blocks cytokine-induced NF-kappa-B activation. Aspirin and sodium salicylate can specifically inhibit IKKß activity in vitro and in vivo by binding to IKKß to reduce ATP binding .

Functions:

The specific activity of IKKß was determined to be 58 nmol /min/mg as per activity assay protocol.

Organism:

Human

Source:

Insect cells

Alias:

IKK2, IKBKB, IKKB, NFKBIKB, FLJ40509, IKK-beta, MGC131801

Gene Symbol:

IKK beta

Gene ID:

N/A

Accession:

NM_001556

Formulation:

50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Solubility:

N/A

Appearance:

Liquid

Molecular Weight (kDa):

105

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

70% - 90%

Concentration:

Shipping Condition :

Dry Ice

Storage Condition:

Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.