Recombinant Human IL15 | Z100459

abm | Recombinant Human IL15 | Z100459

IL-15 has been shown to be produced by human fetal astrocytes and microglial cells in response to IL-1-beta, IFN-gamma, or TNF-alpha and may thus play a role in T cell mediated immune responses in the human central nervous system. IL-15 stimulates proliferation of the established T cell line CTLL-2, CD8(+) memory T cells require IL-15 for proliferation. IL-15 is also able to induce generation of cytolytic cells and LAK cells activity in vitro. IL-15 appears to function as a specific maturation factor for natural killer cells and can mimic the bone marrow microenvironment in vitro, which is required for natural killer cells to acquire lytic functions. IL-15 induces proliferation of mast cells in the absence of functional IL-2 receptor components and utilizes a receptor of 60-65 kDa that is distinct from known components of T cell IL-15 receptors. The IL-15 receptor on mast cells recruits JAK2 and STAT5, instead of JAK1, JAK3 and STAT3 and STAT5 that are activated in T cells. The alpha subunit of the IL-15 receptor (IL-15RA) is structurally related to the alpha subunit of the IL-2 receptor and confers high affinity binding of IL-15 to its receptor. IL-15 binds to the beta and gamma chains of the IL-2 receptor, which are known to be required for ligand internalization and signal transduction.

Functions:

The ED(50) was determined by the dose-dependent proliferation of mouse CTLL-2 cells and was found less than 0.3 ng/mL.

Organism:

Human

Source:

E. coli

Alias:

IL-15, IL-T
Recombinant Human Interleukin-15 (IL15)

Gene Symbol:

IL15

Gene ID:

3600

Accession:

P40933

Formulation:

Recombinant IL-15 was lyophilized from a 0.2 μm filtered PBS solution pH 7.5.

Solubility:

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Appearance:

Lyophilized Powder

Molecular Weight (kDa):

13

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

>95% as determined by SDS-PAGE

Concentration:

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Shipping Condition :

Ambient Temperature

Storage Condition:

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.