Recombinant Human IL18 | Z100479

abm | Recombinant Human IL18 | Z100479

Also known as interferon-gamma-inducing factor (IGIF) and IL-1γ, IL18 is a cytokine that bears structural similarities with members of the IL-1 family of proteins. IL18 is natively synthesized as a proform that gets processed by caspase-1 to generate the bioactive form of IL18. IL18 is one of the pro-inflammatory cytokines that is expressed by a variety of immune and non-immune cells. The activities of IL18 appear to be species specific. An important function of IL18 is the regulation of functionally distinct subsets of T-helper cells required for cell mediated immune responses. IL18 functions as a growth and differentiation factor for Th1 cells and is known to up-regulate the FAS ligand mediated cytotoxic activity of murine natural killer cells. IL18 also partakes in a complex regulatory circuit involved in causing cell death by apoptosis.

Functions:

The ED(50) as determined by the dose-dependent proliferation of human PBMC co-stimulated with IL12 was found to be in the range of 5.0 ng/mL.

Organism:

Human

Source:

E. coli

Alias:

IL-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma
Recombinant Human Interleukin-18 (IL18)

Gene Symbol:

IL18

Gene ID:

3606

Accession:

Q14116

Formulation:

Recombinant Interleukin-18 is supplied as an 0.2 μm filtered PBS solution.

Solubility:

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Appearance:

Lyophilized Powder

Molecular Weight (kDa):

18,5

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

>95% as determined by SDS-PAGE

Concentration:

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Shipping Condition :

Ambient Temperature

Storage Condition:

Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.