Recombinant Human IL1A | Z100405

abm | Recombinant Human IL1A | Z100405

There are two functionally comparable forms of IL1: IL1A and IL1B, that are encoded by two different genes. IL1B is the predominant form in humans while, it is IL1A in mice. Both forms of IL1 bind to the same receptor and therefore show similar, if not identical, biological activities. Latter include but not limited to, stimulation of T-helper cells, promotion, proliferation and synthesis of immunoglobulins and the activation of fibroblasts and NK cells. Unlike IL1B, the IL1A precursor does not require processing before it can bind to the receptor. Monocytes are the main source of secreted IL1B while human keratinocytes are known to express large amounts of IL1A. Recombinant Human IL1A is a non-glycosylated polypeptide of 18 kDa.

Functions:

The ED50 as determined by the dose-dependent stimulation of murine D10S cells was found to be ≤ 0.002 ng/mL

Organism:

Human

Source:

E. coli

Alias:

Hematopoietin-1, Lymphocyte-activating factor, LAF, Mononuclear Cell Factor, MCF, IL1F1
Recombinant Human Interleukin-1 Alpha (IL1A)

Gene Symbol:

IL1A

Gene ID:

3552

Accession:

P01583

Formulation:

Lyophilized from a 0.2 μm filtered solution in Tris and NaCl (pH 8.0)

Solubility:

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Appearance:

Lyophilized Powder

Molecular Weight (kDa):

18

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

>95% as determined by SDS-PAGE

Concentration:

<1.0 EU/μg of recombinant protein as determined by the LAL method

Shipping Condition :

Ambient Temperature

Storage Condition:

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.