Recombinant Human LTA | Z100869

abm | Recombinant Human LTA | Z100869

TNF-beta is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands and signals through TNFR1 and TNFR2. TNF-beta is produced by activated T and B lymphocytes, and has similar activities to TNF-alpha. Like TNF-alpha, TNF-beta is involved in the regulation of various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-beta is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-beta, which effectively anchors the TNF-beta to the cell surface. TNF-beta is cytotoxic to a wide range of tumor cells.

Functions:

The ED(50) was measured in a cytotoxicity assay using mouse L929 cells in the presence of the metabolic inhibitor actinomycin, and was determined to be less than 0.1 ng/mL.

Organism:

Human

Source:

E. coli

Alias:

Tumor necrosis factor ligand superfamily member 1, TNFSF1, Lymphotoxin-alpha, LT-alpha, LTA
Recombinant Human Tumor Necrosis Factor Beta (LTA)

Gene Symbol:

LTA

Gene ID:

4049

Accession:

P01374

Formulation:

TNF-beta is presented as a 0.2 μm filtered PBS solution pH 7.5.

Solubility:

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Appearance:

Lyophilized Powder

Molecular Weight (kDa):

19

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

>95% as determined by SDS-PAGE

Concentration:

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Shipping Condition :

Ambient Temperature

Storage Condition:

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.