Recombinant Human Midkine (NEGF2) | Z102177

abm | Recombinant Human Midkine (NEGF2) | Z102177

Midkine and its functionally-related protein Pleiotrophin (PTN), are heparin-binding neurotrophic factors that signal through the same receptor. Midkine is involved in a number of biological processes including the migration of neutrophils, inflammatory leukocytes, macrophages, neuronal cells, and osteoclasts. Midkine is a basic, non-glycosylated polypeptide that contains five intra-chain disulfide bonds. There is 87% identity between the human and murine Midkine and approximately 50% identity between human Midkine and human PTN with the conservation of all 10 cysteines.

Functions:

The ED(50) was determined by the dose-dependent proliferation of AsPC-1 cells and was found to be <0.5ng/mL.

Organism:

Human

Source:

E. coli

Alias:

MK, NEGF-2

Gene Symbol:

MDK

Gene ID:

4192

Accession:

P21741

Formulation:

Lyophilized from a 0.2 µm filtered solution in PBS

Solubility:

Reconstitute at 0.1 mg/ml in sterile PBS

Appearance:

Lyophilized Powder

Molecular Weight (kDa):

14

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

>95% as determined by SDS-PAGE

Concentration:

< 1.0 EU/μg of recombinant protein as determined by the LAL method.

Shipping Condition :

Ambient Temperature

Storage Condition:

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.