Recombinant Mouse G-CSF (CSF3) | Z200605

abm | Recombinant Mouse G-CSF (CSF3) | Z200605

Granulocyte colony-stimulating factor (G-CSF) is a growth factor and cytokine belonging to the IL-6 family of interleukins. It is produced by a variety of cells such as: endothelial cells, bone marrow stroma, macrophages, fibroblasts, and other immune cells in response to endotoxin activation. G-CSF stimulates the bone marrow to stimulate the survival, proliferation and differentiation of progenitor cells to mature neutrophils while enhancing its activities. It indirectly mobilizes stem cells by downregulating the expression of CXCL12 on marrow osteoblasts and releases neutrophil and monocyte proteolytic enzymes. Mouse G-CSF affects mouse skeletal muscle development by activating proliferation of myoblasts. Human and murine G-CSF shares a 73% amino acid identity and is cross-reactive.

Functions:

The ED50 was 10.0-60.0 pg/mL as determined by a cell proliferation assay

Organism:

Mouse

Source:

E. coli

Alias:

Granulocyte colony-stimulating factor

Gene Symbol:

CSF3

Gene ID:

12985

Accession:

P09920

Formulation:

Lyophilized from a 0.2 µm filtered solution in PBS

Solubility:

Reconstitute in sterile deionized water at 100 µg/mL

Appearance:

Lyophilized Powder

Molecular Weight (kDa):

19,2

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

>95% as determined by SDS-PAGE

Concentration:

<1.0 EU/μg of recombinant protein as determined by the LAL method

Shipping Condition :

Ambient Temperature

Storage Condition:

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.