Recombinant Mouse GM-CSF (CSF2) | Z200077

abm | Recombinant Mouse GM-CSF (CSF2) | Z200077

GM-CSF is a monomeric cytokine with two glycosylation sites that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. CSF2 is secreted together with other factors by T cells and macrophages following cell activation by antigens or mitogens. The signalling ‘GM-CSF:receptor’ complex is a dodecamer of two hexamers of two alpha, two beta, and two ligand subunits. While non-glycosylated and glycosylated GM-CSF show similar activities in vitro, fully glycosylated GM-CSF is biologically more active in vivo. Recombinant Mouse CSF2 is a non-glycosylated, monomeric protein.

Functions:

The ED50 as determined by the dose-dependent stimulation BaF3 cells was < 0.1 ng/mL

Organism:

Mouse

Source:

E. coli

Alias:

GMCSF, Granulocyte-macrophage colony-stimulating factor, CSF2
Recombinant Mouse Granulocyte Macrophage Colony Stimulating Factor (CSF2)

Gene Symbol:

CSF2

Gene ID:

12981

Accession:

P01587

Formulation:

Lyophilized from 0.2 μm filtered solution in Tris, pH 7.5 and NaCl

Solubility:

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Appearance:

Lyophilized Powder

Molecular Weight (kDa):

14

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

>95% as determined by SDS-PAGE

Concentration:

< 1.0 EU/μg of recombinant protein as determined by the LAL method.

Shipping Condition :

Ambient Temperature

Storage Condition:

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.