RNase III, E.coli | E020

abm | RNase III, E.coli | E020

RNase III, E.coli is an endoribonuclease that specifically digests dsRNA into dsRNA fragments of 12-15 base pairs that have two or three nucleotide 3´-overhangs with 5' phosphate and 3' hydroxyl termini.

Concentration:

1 U/μl

Form:

Enzyme supplied with 10X Reaction Buffer

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

One unit is defined as the amount of RNase III, E. coli that is required to hydrolyze 1 nmol of poly(A):poly(U) dsRNA in 30 minutes at 37°C in 1X RNase III, E.coli Reaction Buffer.

This product is distributed for laboratory research only. Caution: Not for diagnostic use.

Application:

Restriction enzyme digestion of double-stranded DNA

Components:

HindIII enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)

Inactivation:

80°C for 20 min

Source:

An E. coli strain that carries the cloned and modified HindIII gene from Haemophilus influenzae Rd (ATCC 51907). The HindIII enzyme recognizes and cuts the sequence A^AGCTT.

Shipping conditions:

Dry ice

Storage conditions:

Store at -20°C.

Storag Buffer:

50 mM Tris-HCl (pH 7.5), 0.5M NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton® X-100 and 50% (v/v) Glycerol.