Description
abm | RNase R | E049
RNase R is an E. coli exoribonuclease which exhibits 3'-to-5' exonuclease activity, efficiently digesting nearly all linear RNA species. This enzyme does not digest circular, lariat, or double stranded RNA with short 3’ overhangs (less than seven nucleotides). As such, this enzyme is ideally suited to the study of lariat RNA produced by traditional splicing, as well as circRNAs which arise through back-splicing. By removing linear RNAs from cellular or RNA extracts, RNase R greatly facilitates the identification of circular species through RNA-sequencing. This enables researchers to probe the landscape of splicing events with greater depth.
Concentration:
10 U/μl
Form:
Enzyme supplied with 10X Reaction Buffer.
Alternate Name:
N/A
Reaction Buffer:
200 mM Tris-HCl, 1 M KCl, 1 mM MgCl2, pH 7.5
Reaction:
Use 1X RNase R Reaction Buffer and incubate at 37°C.
Notes:
1) If degradation is inefficient, use a slightly higher incubation temperature (40-45°C) and supplement additional enzyme partway (e.g. 0.5 µl after 1 hour) through the procedure. The higher temperature is particularly useful for degrading highly structured linear RNAs, such as rRNAs. Do not exceed 45°C or incubate over 3 hours, as this may lead to non-enzymatic RNA degradation.
2) Magnesium at concentrations of 0.1-1.0 mM is required for optimal activity. If EDTA is present, compensate by adding MgCl2 to 1.0 mM final.
3) RNase R exhibits low activity on tRNA, rRNA and other highly structured RNAs, for which the 3’ end is double stranded with a short 3’ overhang. These RNA species can stall the enzyme and result in greatly reduced activity. For best results, removal of rRNA from total RNA extracts is highly recommended.
One unit is defined as the amount of RNase R that converts 1 µg of poly(A) into acid-soluble nucleotides in 10 minutes at 37°C.
This product is distributed for laboratory research only.
Caution: Not for diagnostic use.
Application:
Components:
N/A
Inactivation:
N/A
Source:
N/A
Shipping conditions:
Ice Packs
Storage conditions:
Store all components at -20 °C. Avoid repeated freeze-thaw cycles of all components to retain maximum performance. All components are stable for 1 year from the date of shipping when stored and handled properly.
Storag Buffer:
50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.
1 Review
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Easy
Circular RNA research needs enrichment. One needs to get rid of all RNA than is non circular mRNA tRNAs are all cut away From all supplier of RNAse R only ABM is the producer of RNAse R The price from ABM through Gentaur is the lowest price to get the unique RNAse R to your lab. We can supply more than 40 publications with ABM RNAse R We will have our own circular RNA Library made with this RNAse R. We will release this library in september 2024. Gentaur ABM is your first source for circular DNA Libraries. Technical support about circular is given by phone through Gentaur the sales team.
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