Ryanodine Receptor 1 Stable HEK293 Cell Line | T3037

abm | Ryanodine Receptor 1 Stable HEK293 Cell Line | T3037

Ryanodine receptors (RyR) are intracellular calcium channels located in the sarcoplasmic reticulum and the endoplasmic reticulum, and they mediate the release of calcium ions during muscle contractions. RyR are structurally and functionally similar to the inositol 1,4,5-triphosphate receptors (IP3R), which is responsible in initiating intracellular calcium signaling pathways. Mutations to the receptor have been observed in numerous diseases states. The Ryanodine Receptor 1 Stable HEK293 Cell Line stably expresses functional RyR1 calcium release channels, thus providing a valuable tool for pharmacological investigations in the function and mechanisms of calcium release channels activity, and in depth biophysical studies of the RyR1 receptor.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

36 - 46 hours

Seeding Density:

30,000 - 60,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Rabbit Ryanodine Receptor 1 (RyR1)

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Hygromycin B was used for selection.
2) Western blot and immunofluorescence analysis was used to confirm the expression of RyR1

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C