SEC Component FLAG-AF1 Stable Expressing HeLa S3 Cell Line | T6390

abm | SEC Component FLAG-AF1 Stable Expressing HeLa S3 Cell Line | T6390

HeLa S3 cells are derived from the parental HeLa cell line. To establish this stable cell line, mCAT1 was transfected into the HeLa S3 cells using FuGene HD. The SEC component FLAG-AF1 was then transduced into the HeLa-S3-mCAT1 cells via Ecotropic retrovirus system. This cell line is G418 and hygromycin resistant and can be used in DNA transcription studies.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Cervix

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 34 hours

Seeding Density:

40,000 - 50,000 cells/cm²

Markers:

G418 resistant (ecotropic retroviral infection with mCAT1) Hygromycin resistant

Applications:

For Research Use Only

Doner Gender:

Female

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) PCR; 2) Immunoblotting

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C