SIAT1 Stable Expressing MDCK (MDCK-SIAT1) Cell Line | T6999

abm | SIAT1 Stable Expressing MDCK (MDCK-SIAT1) Cell Line | T6999

The SIAT1 Stable Expressing MDCK (MDCK-SIAT1) Cell Line was generated by stable transfection of MDCK cells with the cDNA of human 2,6-sialtransferase (SIAT1). When compared to the parental MDCK cells, the MDCK-SIAT1 is able to express increased levels of α-2,6-linked sialic acid receptors for isolation of circulating influenza virus. The SIAT1 Stable Expressing MDCK (MDCK-SIAT1) Cell Line is useful in isolation and propagation of human influenza viruses for observation, diagnostic, and research purposes.

Biosafety:

II

Organism:

Canine

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

21 - 31 hours

Seeding Density:

15,000 - 20,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Transfection of MDCK cells with the cDNA of human 2,6-sialtransferase (SIAT1)

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Isolation and passaging of influenza virus

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C