SLC35A1 CRISPR Stable Knockout CHO-S Cell Line | T6040

abm | SLC35A1 CRISPR Stable Knockout CHO-S Cell Line | T6040

SLC35A1 encodes a CMP-sialic acid transporter located within the membrane of the Golgi apparatus. CHO-S cells isolated from Chinese Hamster ovary were transfected with CRISPR/Cas9 plasmids which target SLC35A1 to generate the SLC35A1 CRISPR Knock Out CHO-S Cell Line (Hamster). This sialic acid-deficient cell line is useful for research focusing on SLC35A1 as it functions in transporting nucleotide sugars into the Golgi where glycosyltransferases function. The SLC35A1 CRISPR Knock Out CHO-S Cell Line (Hamster) is also useful in characterization of anti-glycopeptide monoclonal antibodies.

Biosafety:

II

Organism:

Chinese hamster

Source Organ:

Ovary

Growth Properties:

Suspension

Morphology:

Round

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

Transfecting CRISPR/Cas9 plasmids which target SLC35A1

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is CHO-S-SFM II medium Thermo Fisher Scientific Inc). Recommend to add Penicillin/Streptomycin Solution

Preservation:

N/A

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C