Stable EGFR and Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B1B2F3) Cell Line | T3184

abm | Stable EGFR and Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B1B2F3) Cell Line | T3184

Stable EGFR and Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B1B2F3) Cell Line stably expresses EGFR and Erbb2 (trypsine protein kinase receptors). It is a unique cell line in which conditional knockdown expression of the EGFR and the Erbb2 is facilitated by the presence of Cre virus due to the presence of the loxP insertion from the mice. Available at abm, Stable Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B2F6) Cell Line (T3185) and Stable EGFR Conditional Knockdown Mouse Skin Keratinocyte (Egfr2) Cell Line (T3186).

Biosafety:

II

Organism:

Egfr^fl/fl/Erbb2^fl/fl FVB/N Mice

Source Organ:

Skin

Growth Properties:

Adherent

Morphology:

Cobblestone

Clones:

N/A

Passage Number:

N/A

Population Doner:

28 - 38 hours

Seeding Density:

15,000 - 20,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

EGFR and Erbb2, conditional knockdown of EGFR and Erbb2 when cells are infected with the Cre recombinase-expressing adenovirus

Induction:

N/A

Overexpression:

EGFR and Erbb2, conditional knockdown of EGFR and Erbb2 when cells are infected with the Cre recombinase-expressing adenovirus

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow XI medium available at abm

Preservation:

N/A

Quality Control:

1) Immunofluorescence staining was done to assess expression of keratinocyte markers; 2) Infection with Ad-Cre recombinase to assess conditional knockdown of EGFR and Erbb2.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C