Stable EGFR Conditional Knockdown Mouse Skin Keratinocyte (Egfr2) Cell Line | T3186

abm | Stable EGFR Conditional Knockdown Mouse Skin Keratinocyte (Egfr2) Cell Line | T3186

Stable EGFR Conditional Knockdown Mouse Skin Keratinocyte (Egfr2) Cell Line is a unique cell line in which conditional knockdown of the EGFR (epithelial growth factor receptor) is facilitated by the presence of Cre virus. Available at abm, Stable EGFR and Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B1B2F3) Cell Line (T3184) and Stable Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B2F6) Cell Line (T3185).

Biosafety:

II

Organism:

Egfr^fl/fl FVB/N mice

Source Organ:

Skin

Growth Properties:

Adherent

Morphology:

Cobblestone

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

Conditional knockdown of EGFR when cells are infected with the Cre recombinase-expressing adenovirus

Induction:

N/A

Overexpression:

EGFR

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow XI medium available at abm

Preservation:

N/A

Quality Control:

1) Immunofluorescence staining was done to assess expression of keratinocyte markers; 2) Infection with Ad-Cre recombinase to assess conditional knockdown of EGFR.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C