Stable Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B2F6) Cell Line | T3185

abm | Stable Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B2F6) Cell Line | T3185

Stable Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B2F6) Cell Line stably expresses Erbb2 (tryosine protein kinase receptor). It is a unique cell line in which conditional knockdown expression of the Erbb2 is facilitated by the presence of Cre virus due to the presence of the loxP insertion from the mice. Available at abm, Stable EGFR and Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B1B2F3) Cell Line (T3184) and Stable EGFR Conditional Knockdown Mouse Skin Keratinocyte (Egfr2) Cell Line (T3186).

Biosafety:

II

Organism:

Erbb2^fl/flFVB/N Mice

Source Organ:

Skin

Growth Properties:

Adherent

Morphology:

Cobblestone

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

Conditional knockdown of Erbb2 when cells are infected with the Cre recombinase-expressing adenovirus

Induction:

N/A

Overexpression:

Erbb2, conditional knockdown of Erbb2 when cells are infected with the Cre recombinase-expressing adenovirus

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow XI medium available at abm

Preservation:

N/A

Quality Control:

1) Immunofluorescence staining was done to assess expression of keratinocyte markers; 2) Infection with Ad-Cre recombinase to assess conditional knockdown of Erbb2.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C