Stable HA-D2LR-RLuc8 Mutant 5 (RRR-ERE) Expressing HEK-293 Cell Line | T6120

abm | Stable HA-D2LR-RLuc8 Mutant 5 (RRR-ERE) Expressing HEK-293 Cell Line | T6120

HA-D2LR-RLuc8 Mutant 5 (RRR-ERE) Expressing HEK-293 cells are derived from HEK-293 cells transiently transfected with the mutant 5 (RRR-ERE) long form of the D2 dopamine receptor (D2L-R). In this mutated construct, arginine (R) residues in human HA-D2L-R/RLuc8 at positions 267 and 269 were mutated to glutamic acid (E). Constructs were C-terminally tagged with RLuc8 enabling monitoring of total receptor expression. These cells are suitable for research concerning the localization, activity, and expression of this alternatively spliced, D2 dopamine receptor.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

Transfected with HA-D2LR-RLuc8 Mutant 5 using Lipofectamine

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

Not disclosed

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: 92% , 8% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Receptor expressing clones were selected by geneticin (1 mg/ml); 2) Individual clones were isolated via the cloning ring technique/single clone isolation by dilution; 3) Luminescence and fluorescence measurements; 4) ELISA

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C