Stable HEK293 Expressing Rpn11-HTBH Cell Line | T6007

abm | Stable HEK293 Expressing Rpn11-HTBH Cell Line | T6007

Stable HEK293 Expressing Rpn11-HTBH Cell Line was generated through infecting HEK293 cells with retrovirus carrying the pQCXIP-hRpn11-HTBH. Human Rpn11, or the 26S proteasome regulatory subunit rpn11, is the essential deubiquitinase (DUB) of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. HTBH-tag on the C-terminus of the non-ATPase subunit of the human Rpn11 allows for the effective and rapid isolation and purification of the human 26S proteasome complex.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

26 - 34 hours

Seeding Density:

30,000 - 35,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Human Rpn11-HTBh through transduction with replication incompetent retrovirus carrying pQCXIP-hRpn11-HTBH

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1) Human Rpn11-HTBH expressing cells were selected via puromycin (1–2μg∕mL), 2) Immunoblotting was preformed to detect HTBH tag, 3) Isolated human 26S proteasome complex was analyzed via 1D SDS-PAGE.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C