Stable HsTRPML1 (V446L):YFP Expressing HEK293 Cell Line | T6373

abm | Stable HsTRPML1 (V446L):YFP Expressing HEK293 Cell Line | T6373

HEK293 cells were transfected with pcDNA3.1 construct carrying HsTRPML1 (V446L):YFP and were selected under the presence of G418 to create the Stable HsTRPML1 (V446L):YFP Expressing HEK293 Cell Line to study endolysosomal ion channels.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

30 - 40 hours

Seeding Density:

30,000 - 50,000 cells/cm²
* Please note that cells may take up to 72 hours to attach post-thaw

Markers:

TRPML1 V446L mutant, YFP

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C