Stable Human Hepatic Stellate Expressing DDX5-WT Cell Line | T6001

abm | Stable Human Hepatic Stellate Expressing DDX5-WT Cell Line | T6001

Stable Human Hepatic Stellate Expressing DDX5-WT Cell Line is derived from the spontaneously-immortalized human stellate cell line (LX-2) in which was infected with the non-replicating lentivirus carrying the FLAG-tagged DDX5 WT vector. The cells were selected for via 500 µg/ml Zeocin.

Biosafety:

II

Organism:

Human

Source Organ:

Liver

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

collagen type I and α-SMA

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

DDX5 (DEAD box polypeptide 5) via transduction of lentiviral vector

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Selection with 500 µg/ml Zeocin, 2) DAPI stain used to identify DDX5 expression. 3) Western blot preformed to identify presence of DDX5-WT, collagen type I, and α-SMA expression

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C