Stable MEF Raf1 FF-FF Knockin Cell Line | T6107

abm | Stable MEF Raf1 FF-FF Knockin Cell Line | T6107

Raf is a crucial component of the Ras/Raf/MEK/ERK signalling pathway. This signalling pathway is essential for proper regulation of gene expression, cell cycle progression, and apoptosis. Many cancers exhibit disregulation or abnormal activity of this pathway, making it a potential therapeutic target. Stable MEF Raf1 FF/FF Knockin Cells were obtained from knock-in mouse embryos carrying inactive Raf-1FF/FF mutant.

Biosafety:

II

Organism:

Mouse (M. musculus)

Source Organ:

Embryo

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

20,000 - 40,000 cells/cm²

Markers:

Raf1 Y340F Y341F inactive mutant. Vector: pZIPTEX, neomycin resistance

Applications:

For Research Use Only

Doner Gender:

Female

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) FACS; 2)Western blot; 3) Raf kinase cascade assay; 4) MBP kinase assay

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C