Stable ΔN-IκBα Expressing HOS-DS-Red (HOS-DS-Red-ΔN-IκBα) Cell Line | T6109

abm | Stable ΔN-IκBα Expressing HOS-DS-Red (HOS-DS-Red-ΔN-IκBα) Cell Line | T6109

Human Osetosarcoma (HOS) cells were transfected using electorporation with the piggyBac transposon and plasmid carrying a transposase gene to stably express a degradation resistant I-κBα (∆N-I-κBα) under the control of a SV40 promoter. Successful clones were selected for through puromycin resistance. As a result of the constitutive repression of NF-κB by the degradation resistant inhibitor ∆N-I-κBα, these cell are resistant to senescence caused by infection with human T-lymphotropic virus type 1 (HTLV-1). Thus, HOS-DS-Red/ΔN-IκBα cells can be productively infected by HTLV-1 and can spread HTLV-1 to susceptible cells. This line also contains a reporter cassette containing 18 copies of Tax-inducible HTLV-1 21bp repeat, the HTLV-1 TATA element, complete R region, and part of the U5 sequence fused with a gene for DS-Red fluorescence protein (DS-Red) so that upon infection of cells with HTLV-1, the DS-Red protein is expressed. It is suggested that this cell line can be used to study of HTLV-1 replication in cell culture.

Biosafety:

II

Organism:

Human

Source Organ:

Bone

Growth Properties:

Adherent

Morphology:

Bipolar

Clones:

N/A

Passage Number:

N/A

Population Doner:

30 - 40 hours

Seeding Density:

20,000 - 30,000 cells per cm².

Markers:

puromycin

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III available at abm

Preservation:

N/A

Quality Control:

1) Verified the expression of RFP upon HTLV-1 infection via imaging (Figure 1).2) Showed cells could spread from one HOS line to another as well as to a T-cell reporter line by imaging RFP expression and measuring Luciferase activity from the report lines (Figure 5).

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C