Stable Rat Expressing HA-tagged SCL26A9 Thyroid Epithelial (SLC26A9-FRT) Cell Line | T3188

abm | Stable Rat Expressing HA-tagged SCL26A9 Thyroid Epithelial (SLC26A9-FRT) Cell Line | T3188

Stable Rat Expressing HA-tagged SCL26A9 Thyroid Epithelial (SLC26A9-FRT) Cell Line expresses the human HA-tagged SCL26A9 Cl- channel. Studies speculate that these channels may become a potential therapeutic target for the deficient CFTR-mediated Cl- secretion in cystic fibrosis. The cell line offers a valuable renewable in vitro model to study the effects of the SCL26A9 Cl- channels.

Biosafety:

II

Organism:

Fischer rat

Source Organ:

Thyroid

Growth Properties:

Adherent

Morphology:

Epithelial-Like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Infection with Replication-defective retroviral particles to express human HA-tagged SCL26A9 gene

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line Coon’s F12 modified medium F0855, Biochrom). To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 5% and Penicillin/Streptomycin Solution

Preservation:

N/A

Quality Control:

1) 1 µg/ml puromycin for selection; 2) Western blot used to determine the presence of SCL26A9

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C