Stable TALEN hsMgat1-GnT-1 Knock Out CHO-S Cell Line | T6110

abm | Stable TALEN hsMgat1-GnT-1 Knock Out CHO-S Cell Line | T6110

MGAT1 or GnT-1 is essential for the synthesis of hybrid and complex N-glycans. CHO-S cells isolated from Chinese Hamster ovary were transfected with TALEN plasmid which target hsMgat1 to generate the Stable TALEN hsMgat1/GnT-1 Knock Out CHO-S Cell Line. This glycan-deficient cell line is useful for research on MGAT1 and its role in N-glycan synthesis. The Stable TALEN hsMgat1/GnT-1 Knock Out CHO-S Cell Line is also useful in characterization of anti-glycopeptide monoclonal antibodies.

Biosafety:

II

Organism:

Chinese hamster

Source Organ:

Ovary

Growth Properties:

Suspension

Morphology:

Round

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

Transfection using TALEN plasmid targeting hsMgat1

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is CHO-S-SFM II medium Thermo Fisher Scientific Inc). Recommend to add Penicillin/Streptomycin Solution

Preservation:

N/A

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C