T2R7 Expressing HEK293T Cell Line | T6139

abm | T2R7 Expressing HEK293T Cell Line | T6139

T2R7 Expressing HEK293T Cell Line are human kidney cells which stably express the bitter taste receptor T2R7. T2R7 Expressing HEK293T Cell Line was established by transfecting HEK293T cells with pcDNA 3.1/Hygro (+) expression vector that had the synthetic TAS2R7 gene cloned into it. The T2R7 Expressing HEK293T Cell Line is suitable for research into taste receptors and bitter compounds which can activate those receptors.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

16 - 26 hours

Seeding Density:

50,000 - 10,000 cells/cm²

Markers:

Hygromycin resistance, expression of FLAG sequence at N-terminus of bitter taste receptors

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is PriGrow IV medium available from abm TM004). To make the completed growth medium, add the following components to the base medium: Fetal Bovine Serum TM999) to a final concentration of 10% and Penicillin/Streptomycin G255) to a final concentration of 1%. Change medium every 2 days and split cells accordingly when the cells are at 80-90% confluent.
Carbon dioxide CO₂): 5%, Temperature: 37.0°C.

Preservation:

N/A

Quality Control:

Expression of FLAG sequence at N-terminus of receptors analyzed by flow cytometry.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C