ApaI | E063
- 5 to 7 Days Shipment
abm | ApaI | E063
Buffer 1: 25%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes.
1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) Incubate at 25°C. ApaI has 100% activity but a reduced half-life of 30 minutes if incubated at 37°C. ApaI is inhibited by salt concentrations higher than 50mM.
Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive
10mM Tris pH (7.5), 50mM KCl, 1mM DTT, 0.1mM EDTA, and 50% (v/v) Glycerol.
10000 U (500 µl)