Atg5 Knockout Immortalized Mouse Kidney Epithelial Cell Line | T3027

abm | Atg5 Knockout Immortalized Mouse Kidney Epithelial Cell Line | T3027

Autophagy is a catabolic process in which proteins and cellular organelles are degraded under metabolic stress through the lysosomal pathway. Essential autophagy-related genes (i.e. Atg 5 and Atg 7) play an important role in mammalian development, survival and maintaining metabolism and homeostasis. The Atg5 Knockout Immortalized Mouse Kidney Epithelial Cells (Atg5^-/- iBMK) is derived from stable transformation of E1A and p53DD into primary kidney epithelial cells from mice deficient for Atg5. Together with the wild-type iBMK

Biosafety:

II

Organism:

Baby C57B/6 mouse with Atg5^-/- genotype

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Cuboidal

Clones:

7.1

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

E-cadherin, CK8 and CK18

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line. 2. Western blot was used to check epithelial markers E-cadherin and cytokeratin expression. 3. PCR genotyping of the pup tail and western blot analysis were performed to verify the genotype of the cell line.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C