Atg7 Knockout Immortalized Mouse Kidney Epithelial Cell Line | T3028
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abm | Atg7 Knockout Immortalized Mouse Kidney Epithelial Cell Line | T3028
Autophagy is a catabolic process in which proteins and cellular organelles are degraded under metabolic stress through the lysosomal pathway. Essential autophagy-related genes (i.e. Atg 5 and Atg 7) play an important role in mammalian development, survival and maintaining metabolism and homeostasis. The Atg7 Knockdown Immortalized Mouse Kidney Epithelial Cells (Atg7-/- iBMK) is derived from stable transformation of E1A and p53DD into primary kidney epithelial cells from mice deficient for Atg7. Together with the wild-type iBMK
Baby C57B/6 mouse with Atg7-/- genotype
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
E-cadherin, CK8 and CK18
For Research Use Only
The base medium for this cell line is Prigrow III medium available at
1. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line. 2. Western blot was used to check epithelial markers E-cadherin and cytokeratin expression. 3. PCR genotyping of the pup tail was performed to verify the genotype of the cell line.
10⁶ cells/1.0 ml