Bak Knockout Immortalized Mouse Kidney Epithelial Cell Line | T3025

abm | Bak Knockout Immortalized Mouse Kidney Epithelial Cell Line | T3025

Key regulators of apoptosis, such as members of the BCL-2 family, have long been linked to the development of cancer. The Bak Knockout Immortalized Mouse Kidney Epithelial Cells (bak^-/- iBMK) is derived from stable transformation of E1A and p53DD into primary baby kidney epithelial cells from mice deficient for Bak. Together with the wild-type iBMK (T0082), Bax knockout iBMK (bax^-/- iBMK, T3024) and Bak and Bax Double Knockdown iBMK (Bak^-/-/Bax^-/-iBMK, T3026), these cell lines are suitable in studying the relationships between BCL-2 family and cancer progression, prognosis and significance to treatment.

Biosafety:

II

Organism:

Baby C57B/6 mouse with bak^-/-, bax^+/- genotype

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Cuboidal

Clones:

K1

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

E-cadherin, CK8 and CK18

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line. 2. Western blot was used to check epithelial markers E-cadherin and cytokeratin expression. 3. PCR genotyping of the pup tail was performed to verify the genotype of the cell line.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C