abm drug discovery
c-Kit-D816YStable Ba-F3 Cell Line | T3054
- SKU:
- T3054
- Availability:
- 5 to 7 Days Shipment
Description
abm | c-Kit-D816YStable Ba-F3 Cell Line | T3054
c-Kit or stem cell factor (SCF) receptor is a type III tyrosine kinase receptor that has a major role in the hematopoietic system. Gain-of-function mutations of the receptor have been observed in malignancies such as myeloid leukemia, gastrointestinal stromal tumors, and testicular carcinoma. While loss-of-function mutations were found in defects relating to hematopoiesis, melanogenesis, and gametogenesis. The c-Kit/D816Y-expressing Ba/F3 cells (with GNNK- isoform) has a substitution of the aspartic acid residue at position 816 to a tyrosine. The cells are resistant to Imatinib (Gleevec), an inhibitor of the c-Kit juxtamembrane mutations. Together with the wild-type c-Kit stable Ba/F3 cells (
Biosafety:
I
Organism:
Mouse
Source Organ:
Marrow
Growth Properties:
Suspension
Morphology:
Round
Clones:
N/A
Passage Number:
N/A
Population Doner:
N/A
Seeding Density:
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers:
N/A
Applications:
For Research Use Only
Doner Gender:
N/A
Donor Ethnicity:
N/A
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
Transfected with pMSCVpuro vector carrying mutant D816Y human c-Kit construct
Freeze Thaw:
N/A
Propagation:
The base medium for this cell line is Prigrow II medium available at abm
Preservation:
N/A
Quality Control:
1) Immunoprecipitation and western blotting for presence of c-Kit; 2) PCR analysis
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C