c-Kit-WT Stable Ba-F3 (with GNNK+ isoform) Cell Line | T3042

abm | c-Kit-WT Stable Ba-F3 (with GNNK+ isoform) Cell Line | T3042

c-Kit or stem cell factor (SCF) receptor is a type III tyrosine kinase receptor that has a major role in the hematopoietic system. The c-Kit wild-type-expressing Ba/F3 cells (with GNNK+ isoform) are ideal in studies in relation to c-Kit function, signaling pathways, and biological activities. The GNNK+ isoform is the outcome of the c-Kit gene alternative splicing sites resulting in different isoforms of the tetrapeptide GNNK. It will proliferate in the presence of the ligand SCF, and activate downstream signal transductions. When compared to GNNK- isoforms (

Biosafety:

I

Organism:

Mouse

Source Organ:

Marrow

Growth Properties:

Suspension

Morphology:

Round

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Transfected with pMSCVpuro vector carrying wild type human c-Kit construct

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available from ABM TM002). To make the completed growth medium, add the following components to the base medium: 10 ng/ml recombinant murine IL-3, 10% fetal bovine serum TM999), and 1% Penicillin/Streptomycin G255). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C.

Preservation:

N/A

Quality Control:

1) Immunoprecipitation and western blotting for presence of c-Kit and isoform GNNK+; 2) Cell survival assay with stem cell factor (SCF)

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C