c-Kit-Y570F Stable Ba-F3 Cell Line | T3044

abm | c-Kit-Y570F Stable Ba-F3 Cell Line | T3044

c-Kit or stem cell factor (SCF) receptor is a type III tyrosine kinase receptor that has a major role in the hematopoietic system and has nine key tyrosine residues. Y570 residue, alongside with the Y568 residue, is involved in recruiting signaling molecules, and is part of the autoinhibition process of enzyme activity. c-Kit/Y570F-expressing Ba/F3 cells (with GNNK- isoform) has a Y-to-F mutation at position 570. The GNNK- isoform is the outcome of the c-Kit gene alternative splicing sites, resulting in different isoforms of the tetrapeptide GNNK, and is the main transcript found in human malignancies. It will proliferate in the presence of the ligand SCF, and activate downstream signal transduction. Together with the wild-type c-Kit stable Ba/F3 cells (

Biosafety:

I

Organism:

Mouse

Source Organ:

Marrow

Growth Properties:

Suspension

Morphology:

Round

Clones:

N/A

Passage Number:

N/A

Population Doner:

16 - 26 hours

Seeding Density:

75,000 - 100,000 cells/mL

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Transfected with pMSCVpuro vector carrying mutant Y568F human c-Kit construct

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

N/A

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C