c-Kit-Y823F Stable Ba-F3 Cell Line | T3058

abm | c-Kit-Y823F Stable Ba-F3 Cell Line | T3058

c-Kit or stem cell factor (SCF) receptor is a type III tyrosine kinase receptor that has a major role in the hematopoietic system and has nine key tyrosine residues. c-Kit/Y823F-expressing Ba/F3 cells (with GNNK- isoform) has a Y-to-F mutation at position 823. The GNNK- isoform is the outcome of the c-Kit gene alternative splicing sites, resulting in different isoforms of the tetrapeptide GNNK, and is the main transcript found in human malignancies. It will proliferate in the presence of the ligand SCF, and activate downstream signal transduction. Together with the wild-type c-Kit stable Ba/F3 cells (

Biosafety:

I

Organism:

Mouse

Source Organ:

Marrow

Growth Properties:

Suspension

Morphology:

Round

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Transfected with pMSCVpuro vector carrying mutant Y823F human c-Kit construct

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

N/A

Quality Control:

1) Immunoprecipitation and western blotting for presence of c-Kit; 2) PCR analysis

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C