abm drug discovery
Cas9 Expressing HCT116 Cell Line | T3263
- SKU:
- T3263
- Availability:
- 5 to 7 Days Shipment
Description
abm | Cas9 Expressing HCT116 Cell Line | T3263
HCT116 cells stably expressing Cas9
Biosafety:
II
Organism:
Human
Source Organ:
Colon
Growth Properties:
Adherent
Morphology:
Epithelial-Like
Clones:
N/A
Passage Number:
N/A
Population Doner:
18 - 36 hours
Seeding Density:
N/A
Markers:
N/A
Applications:
N/A
Doner Gender:
N/A
Donor Ethnicity:
N/A
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
N/A
Freeze Thaw:
1. Pre-warm complete media in a 37°C waterbath.
2. Remove the cryopreserved vial from the liquid nitrogen storage tank.
3. Thaw the cells quickly by placing the lower half of the vial into the 37°C water bath and remove after 60 seconds. There should still be a few ice crystals left after thawing. It is important not to over-thaw the cryovials as the presence of DMSO is toxic to the cells.
4. Re-suspend the cells in the vial and transfer the cell suspension into a 15mL sterile conical tube containing 5mL complete media.
5. Centrifuge cells at 200x g for 3 minutes to pellet.
6. Aspirate out the media, leaving cell pellet undisturbed.
7. Re-suspend pellet in fresh culture medium and plate in new culture vessel.
8. Incubate cultures at 37°C, 5% CO2.
Propagation:
The base medium for this cell line is Prigrow CI Medium available at abm
Preservation:
N/A
Quality Control:
1) Drug selection
2) Western Blot analysis for Cas9 transgene expression
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C