Cas9 Expressing RCS Cell Line | T3290
- 5 to 7 Days Shipment
abm | Cas9 Expressing RCS Cell Line | T3290
Swarm rat chondrosarcoma (RCS) cells were derived from neoplasm in rat spine and is a model for chondrosarcoma tumor development. The Cas9 expressing RCS Cell Line was established through co-transfection with hCas9_P2A_mCherry_T2a_Puro and pOG44 vector. The generation of the Cas9 expressing RCS cell line allows users to generate targeted mutations in genes via transfection or lentiviral transfection with single-guide RNAs (sgRNAs). This cell line is a valuable research tool which enables users endless possibilities to design their desired cell line for the study of chondrocytes and gene function.
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
The base medium for this cell line is Prigrow III medium available at abm
1) Puromycin selection
2) Western Blot analysis for Cas9 transgene expression
10⁶ cells/1.0 ml