Cas9 Nuclease NLS Protein | K151
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abm | Cas9 Nuclease NLS Protein | K151
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR). The Cas9 nuclease from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency in vivo compared to other variants. Cas9 Nuclease NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.
Lyophilized powder. Enzyme supplied with 10X Reaction Buffer.
spCas9, spyCas9, CRISPR-associated endonuclease Cas9 from Streptococcus pyogenes
This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Store all components at -20°C.
After resuspension in 125 µl nuclease-free dH2O: 10mM Tris pH 7.5, 100mM NaCl, 0.1mM EDTA, 1mM DTT, 1% Sucrose.