CEA-2 Stable MC-38 Cell Line | T6408

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SKU:
T6408
Availability:
5 to 7 Days Shipment
€4,173.00
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Description

abm | CEA-2 Stable MC-38 Cell Line | T6408

MC38 murine colonic adenocarcinoma cells were transduced with a retrovirus containing the human Carcinoembryonic antigen (CEA) gene. The CEA gene expressed in T6408 is a single M, 70,000 glycosylated immunoreactive product. The CEA gene present in T6408 was partially sequenced and found to have a deletion of two of the three repeated domains contained in CEA. Despite the loss of two domains the CEA product expresses epitopes recognized by multiple anti-CEA MAbs This cell line can be used as a model to study reagents against a carcinoma expressing human CEA.

Biosafety:

II

Organism:

Mouse (M. musculus)

Source Organ:

Colon

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

CEA, G418 resistance

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

Use of PriCoatTM T25 Flasks

Preservation:

1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot; 2) Southern blot; 3) Sequencing; 4) Immunofluorescence assay; 5) Immunoprecipitation

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C

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Additional Information

Size:
10⁶ cells/1.0 ml
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