Cytochrome c Stable Knockout Mouse Fibroblast Cell Line | T6305

abm | Cytochrome c Stable Knockout Mouse Fibroblast Cell Line | T6305

Mice harboring a heterozygous somatic cyt c KO allele (cyt cs+/−) with mice harboring a homozygous testis cyt c KO allele (cyt ct−/−) were crossed. We used homozygous cyt ct−/− females for the crosses. Embryonic lethal phenotype was rescued by introducing a ubiquitously expressed somatic cyt c transgene flanked by loxP sites. Crosses of one of the transgenic animals with the cyt cyt−/−cyt cs+/− animals gave rise to F1 mice that when backcrossed produced mice with the cyt ct−/−cyt cs−/− cyt cflox/o genotype, demonstrating that the transgene was able to rescue the embryonic lethal phenotype of the cyt cs KO. Lung fibroblasts (LF) from these animals were cultured and the transgenic cyt c cDNA deleted ex vivo by an adenovirus preparation expressing the Cre recombinase. clones by serial dilution and screened them for the presence of Cyt c via immunocytochemistry or western blot, in which the resultant clone had no detectable Cyt c to generate the Cytochrome c Stable Knockout Mouse Fibroblast Cell Line.

Biosafety:

II

Organism:

Mouse (M. musculus)

Source Organ:

Lung

Growth Properties:

Adherent

Morphology:

Fibroblast

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 hours

Seeding Density:

N/A

Markers:

G418 and hygromycin-resistant

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot; 2) IHC

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C