Description
abm | DpnI | E026
The DpnI restriction enzyme digests DNA at GmeA↓TC sites, requiring N6-methylation of the adenine residue for activity. DNA purified from a dam+ strain will be a substrate for DpnI due to the adenine methylation. DpnI cleaves hemi-methylated dam sites 60 X more slowly than fully methylated.
Concentration:
20 U/μl
Form:
Enzyme supplied with 10X Reaction Buffer
Alternate Name:
N/A
Reaction Buffer:
N/A
Reaction:
N/A
Notes:
One unit is defined as the amount of E. coli DNA Ligase that is required to give 50% ligation of HindIII-digested λ DNA in a total reaction volume of 20 μl in 30 minutes at 16°C in 1X E.coli DNA Ligase Reaction Buffer (concentration of 5′ DNA termini is 0.12 μM [300 μg/ml]).
This product is distributed for laboratory research only.
Caution: Not for diagnostic use .
Application:
N/A
Components:
N/A
Inactivation:
N/A
Source:
E. coli
Shipping conditions:
Dry ice
Storage conditions:
-20°C
Storag Buffer:
10 mM Tris-HCl (pH 7.5), 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 µg/ml BSA, and 50% (v/v) Glycerol.
Discount(%):
30