E-Cre-ERT2 Stable Expressing Rat1 (AD6) Cell Line | T6169
- 5 to 7 Days Shipment
abm | E-Cre-ERT2 Stable Expressing Rat1 (AD6) Cell Line | T6169
This rat cell line expresses a chimeric molecule containing a Δ E-like fragment of Notch1 fused to a vesicular stomatitis virus epitope, the Cre recombinase, and a modified hormone binding domain of the estrogen receptor. The delivery of Cre recombinase depends on any event, including γ-secretase processing, that releases the intracellular domain of Notch1 from the membrane. Nuclear activity of the Cre-ER-T2 chimera depends on the presence of tamoxifen. Reporter construct for the recombinase activity consists of an antibiotic resistance gene (hygromycin). This cell line would be well-suited for research regarding the functions of the Notch receptor, γ-secretase enzyme activity, γ-secretase multiprotein complex, and their involvement in cellular signalling.
Rat (R. norvegicus)
Neomycin resistant if not treated with tamoxifen. Hygromycin resistant if treated with tamoxifen.
For Research Use Only
The base medium for this cell line is Prigrow III medium available at abm
1) Immunofluorescence; 2) Northern blot; 3) DNA sequencing
10⁶ cells/1.0 ml