Description
abm | EcoRV | E059
N/A
Concentration:
20000 U/ml
Form:
N/A
Alternate Name:
N/A
Reaction Buffer:
Buffer 1: 25%, Buffer 2: 100%, Buffer 3: 100%, Universal Buffer: 100%
Click here to view the buffer compatibility chart for restriction enzymes.
Reaction:
1X Universal Restriction Enzyme Reaction Buffer. Incubate at 37°C
Notes:
One unit is defined as the amount of Exonuclease III, E. coli that is required to catalyze the release of 1 nmol of acid soluble nucleotides from double stranded DNA in 30 minutes at 37°C in 1X Exonuclease III Reaction Buffer.
This product is distributed for laboratory research only.
Caution: Not for diagnostic use.
Application:
Restriction enzyme digestion of double-stranded DNA
Components:
AflII enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)
Inactivation:
65°C for 20 min
Source:
An E. coli strain that carries the AflII gene from Anabaena flos-aquae (CCAP 1403/13f). The AflII enzyme recognizes and cuts the sequence C^TTAAG.
Shipping conditions:
Ice Packs
Storage conditions:
-20°C
Storag Buffer:
10mM Tris pH (7.5), 100mM NaCl, 1mM DTT, 0.1mM EDTA, and 50% (v/v) Glycerol.
Discount(%):
30