FGFR1 Stable U2OS Cell Line | T6017

abm | FGFR1 Stable U2OS Cell Line | T6017

FGFR1 Stable U2OS Cell Line can be used as a research tool to investigate ubiquitination of FGFR1, effects with FGF1 stimulation, and pathways involved. Parental U2OS cells were stably transfected with plasmid carrying the human FGFR1 gene. The cells are desirable for further manipulation and used to study the effects.

Biosafety:

II

Organism:

Human

Source Organ:

Bone

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

130 - 140 hours

Seeding Density:

20,000 - 50,000 cells/cm²; Split ratio: 1:3 – 2:3

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

Female

Donor Ethnicity:

Caucasian

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

hFGFR1 plasmid stably transfected into parental U2OS cells

Freeze Thaw:

N/A

Propagation:

This cell line requires the Applied Cell Extracellular Matrix G422) for optimal growth. The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1) Selection with 1 mg/ml G418 in culture; 2) receptor expression level analyzed by immunofluorescence and immunoblotting

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C