GALK1+GALE Stable Knockout HEK293T Cell Line | T6151

abm | GALK1+GALE Stable Knockout HEK293T Cell Line | T6151

The UDP-galactose-4 epimerase (encoded by GALE) and Galactokinase 1 (GALK1) are both major enzymes in the the Leloir pathway, the primary pathway for galactose metabolism. Both enzymes modify the sugar galactose, generating precursors for O-linked glycolsylation. Deficiencies in these genes can lead to Galactosemia: a condition that is caused by the acumulation of galactose in the blood and tissue of individuals that cannot properly metabolize the galactose in their diets. As this cell line is derived from a human source, it may be useful in the study of glycosylation and how it is affected during instances of disease.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

Neomycin

Applications:

For Research Use Only

Doner Gender:

Embryo

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III available from abm

Preservation:

N/A

Quality Control:

1) Confirmed GALK1 sequencing (Figure S2). Tested O-glycosylation levels through enzymatic activity on the substrate SIVmac239 gp120 via Western Blot and SDS gel analysis (Figure 4).

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C