GFP Stably Expressing Jurkat Cell Line | T3920
- 5 to 7 Days Shipment
abm | GFP Stably Expressing Jurkat Cell Line | T3920
GFP Stably Expressing Jurkat Cell Line
40 - 50 hours
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
1. Pre-warm complete media in a 37°C waterbath. 2. Remove the cryopreserved vial from the liquid nitrogen storage tank. 3. Thaw the cells quickly by placing the lower half of the vial into the 37°C water bath and remove after 60 seconds. There should still be a few ice crystals left after thawing. It is important not to over-thaw the cryovials as the presence of DMSO is toxic to the cells. 4. Re-suspend the cells in the vial and transfer the cell suspension into a 15mL sterile conical tube containing 5mL complete media. 5. Centrifuge cells at 1500rpm for 3 minutes to pellet. 6. Aspirate out the media, leaving cell pellet undisturbed. 7. Re-suspend pellet in fresh culture medium and plate in new culture vessel. 8. Incubate cultures at 37°C, 5% CO2.
The base medium for this cell line is PriGrow II medium available at abm
1. Puromycin selection 2. FACS Sorting of very high GFP expressing population.
10⁶ cells/1.0 ml