HIF HRE-Luc-LacZ Stable Expressing LN229 Human Glioblastoma Cell Line | T6295

abm | HIF HRE-Luc-LacZ Stable Expressing LN229 Human Glioblastoma Cell Line | T6295

HIF HRE-Luc/LacZ Stable Expressing LN229 Human Glioblastoma Cell Line expresses hypoxia-responsive dual LacZ/Luciferase reporter to monitor HIF-1 activity. This cell line is useful to study the effects of HIF-1 activity.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Brain

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

30 - 40 hours

Seeding Density:

N/A

Markers:

Hygromycin B resistant

Applications:

For Research Use Only

Doner Gender:

Female

Donor Ethnicity:

Caucasian

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

Note: selection antibiotics should not be added upon thawing or for routine cell propagation. Let cells recover first after thawing, then once they grow well put for 7 days in selection medium. Then grow again in normal medium.
The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) STR

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C