HindIII | E071
- 5 to 7 Days Shipment
abm | HindIII | E071
Buffer 1: 10%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%
View the buffer compatibility chart for restriction enzymes.
1X Universal Restriction Enzyme Reaction Buffer (Cat. No. E204).Incubate at 37°C.
One unit is defined as the amount of enzyme needed to generate 1 μmol of phosphate in 1 minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).
Restriction enzyme digestion of double-stranded DNA
BamHI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)
An E. coli strain that carries the cloned and modified BamHI gene from Bacillus amyloliquefaciens H (ATCC 49763). The BamHI enzyme recognizes and cuts the sequence G^GATCC.
0.1 mM EDTA, 50% Glycerol, 1 mM DTT, 10 mM Tris-HCl, 300 mM NaCl, 500 µg/ml BSA, pH 7.4 @ 25°C.
10000 U (500 µl)