HindIII | E071

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SKU:
E071
Availability:
5 to 7 Days Shipment
€170.00
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Description

abm | HindIII | E071

N/A

Concentration:

20000 U/ml

Form:

N/A

Alternate Name:

N/A

Reaction Buffer:

Buffer 1: 10%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%
View the buffer compatibility chart for restriction enzymes.

Reaction:

1X Universal Restriction Enzyme Reaction Buffer (Cat. No. E204).
Incubate at 37°C.

Notes:

One unit is defined as the amount of enzyme needed to generate 1 μmol of phosphate in 1 minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).

Application:

Restriction enzyme digestion of double-stranded DNA

Components:

BamHI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)

Inactivation:

No

Source:

An E. coli strain that carries the cloned and modified BamHI gene from Bacillus amyloliquefaciens H (ATCC 49763). The BamHI enzyme recognizes and cuts the sequence G^GATCC.

Shipping conditions:

Ice Packs

Storage conditions:

-20°C

Storag Buffer:

0.1 mM EDTA, 50% Glycerol, 1 mM DTT, 10 mM Tris-HCl, 300 mM NaCl, 500 µg/ml BSA, pH 7.4 @ 25°C.

Discount(%):

30

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Additional Information

Size:
10000 U (500 µl)
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